Chunking >100mb file on Flask - flask

Question

I would like to upload files between 100-200mb using Flask on PythonAnywhere (which has a 100mb upload limit). I am trying to implement chunking but am still getting a 413 Request Entity Too Large error and am unsure why. I assume it has to do with using open(large_file) but am unsure how to get around this. Any help would be apprecaited?
All the files in /home/zweiss124/mysite/files that end in .fastq are 100-200mb.
def read_in_chunks(file_object, chunk_size=10000):
while True:
data = file_object.read(chunk_size)
if not data:
break
yield data
def get_uploaded_files():
global uploaded_files
uploaded_files = []
global num_chunks
num_chunks = []
os.chdir("/home/zweiss124/mysite/files")
#for file in shared_dict["filenames"]:
for file in glob.glob("*.fastq"):
f = open(file)
index = 0
offset = 0
headers = {}
all_chunks = ''
ind_num_chunks = 0
for chunk in read_in_chunks(f):
offset = index + len(chunk)
headers['Content-Type'] = 'application/octet-stream'
index = offset
all_chunks+=chunk
ind_num_chunks+=1
num_chunks.append(ind_num_chunks)
uploaded_files.append(all_chunks.split("\n")[:4*shared_dict["num_to_upload"]])
return uploaded_files, num_chunks