Applying Multi Otsu Threshold for my image - computer-vision

Question

I have this image shown below
And, here I am trying to define the threshold to distinguish bimodal class by using the Otsu technique based on intensity and then visualise those in the histogram. So far I have written following codes:
import matplotlib.pyplot as plt
import numpy as np
from skimage import data, io, img_as_ubyte
from skimage.filters import threshold_multiotsu
# Read an image
image = io.imread("Fig_1.png")
# Apply multi-Otsu threshold
thresholds = threshold_multiotsu(image,classes=5)
# Digitize (segment) original image into multiple classes.
#np.digitize assign values 0, 1, 2, 3, ... to pixels in each class.
regions = np.digitize(image, bins=thresholds)
output = img_as_ubyte(regions) #Convert 64 bit integer values to uint8
fig, ax = plt.subplots(nrows=1, ncols=3, figsize=(10, 3.5))
# Plotting the original image.
ax[0].imshow(image, cmap='gray')
ax[0].set_title('Original')
ax[0].axis('off')
# Plotting the histogram and the two thresholds obtained from
# multi-Otsu.
ax[1].hist(image.ravel(), bins=255)
ax[1].set_title('Histogram')
for thresh in thresholds:
ax[1].axvline(thresh, color='r')
# Plotting the Multi Otsu result.
ax[2].imshow(regions, cmap='gray')
ax[2].set_title('Multi-Otsu result')
ax[2].axis('off')
plt.subplots_adjust()
plt.show()
This gives me the following result. Here As you can see Multi-Otsu result is totally black and does not show the two class of object present in the figure.
I choose classes=5 but this is bimodal hence putting classes=3 also giving me the same result.
Any advice on how to correct this? Thanks in advance.